Taking the stress out of blood collection: comparison of field blood-sampling techniques for analysis of baseline corticosterone

Publication Type:Journal Article
Year of Publication:2008
Authors:Arnold, JM, Oswald, SA, Voigt, CC, Palme, R, Braasch, A, Bauch, C, Becker, PH
Journal:Journal of Avian Biology
Volume:39
Issue:5
Date Published:2008
ISBN Number:1600-048X
Keywords:Hirundo, Laridae, Sterna, Sterna hirundo
Abstract:Many ecological studies use stress hormones to assess the condition, health or disturbance levels of wild organisms. Common blood sampling protocols for this research involve trapping individuals and taking blood within three minutes to obtain a “baseline” for analysis of stress hormones (“conventional method”). In some situations it may be difficult to get an accurate measure of baseline values; therefore, alternative sampling techniques may be preferable. We compared corticosterone levels in samples taken via a newly developed, minimally invasive blood sampling technique with corticosterone levels in blood taken via the conventional method. We collected samples from incubating adult common terns Sterna hirundo via blood sucking bugs (Heteroptera, Triatominae) contained in “dummy eggs” (“bug method”) and compared measured corticosterone concentrations to concentrations in blood taken from the same birds using the conventional method. We found no significant differences in mean or variance of baseline corticosterone levels between samples collected via the different methods. This suggests that the bug method offers a viable alternative for hormone sampling.
URL:http://dx.doi.org/10.1111/j.0908-8857.2008.04265.x
Short Title:Journal of Avian Biology
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith