Birds of Indian Subcontinent

Propagation of Virulent and Avirulent Turkey Hemorrhagic Enteritis Virus in Cell Culture

Publication Type:Journal Article
Year of Publication:1982
Authors:Nazerian, K, Fadly, AM
Journal:Avian Diseases
Date Published:1982
ISBN Number:00052086
Keywords:Electron, Meleagris, Meleagris gallopavo, Phasianidae
Abstract:Virulent and apathogenic isolates of turkey hemorrhagic enteritis virus (HEV) were successfully propagated in lymphoblastoid cell lines of turkey origin, whereas spleen and kidney cell cultures from HEV-infected turkeys failed to replicate the virus. The lymphoblastoid cell lines used were MDTC-RP16 and MDTC-RP19, which were previously established from tumors induced by Marek's disease virus in turkeys. Virus replication followed co-cultivation of lymphoblastoid cells with spleen cells from HEV-infected turkeys. Virus replication was demonstrated by immunofluorescence, by agar-gel-precipitin tests, and by electron microscopy. Supernatant fluid of cultures infected with virulent HEV caused death and specific lesions in turkey poults. Poults vaccinated with apathogenic HEV were protected against death and lesions after challenge with pathogenic HEV, which was recovered from infected cultures. The MDTC-RP19 cell line appeared far more susceptible than the MDTC-RP16 cell line to infection with HEV.
Short Title:Avian Diseases
Taxonomic name: 
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