Phytohaemagglutinin injection has a long-lasting effect on immune cells

Publication Type:Journal Article
Year of Publication:2009
Authors:Sarv, T, Hõrak, P
Journal:Journal of Avian Biology
Date Published:2009
ISBN Number:1600-048X
Keywords:Carduelis, Carduelis chloris, Chloris, Chloris chloris, Fringillidae
Abstract:Measurement of phytohaemagglutinin (PHA)-induced skin swelling is the most popular assay of immune function in avian studies. The mechanisms causing swelling have been relatively well studied; however, very little is known about the potential long term physiological effects of PHA. Here we show that injection of PHA into patagium of captive greenfinches Carduelis chloris increases the concentration of heterophils (phagocytic cells of the innate immune response) in the peripheral blood for at least 30 days. Such long-term consequences should be taken into account when using PHA skin test in studies monitoring changes in individual physiological condition and/or immune status.
Short Title:Journal of Avian Biology
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith