On the occurrence of proteolytic activities in ovarian follicles of the stick insect Carausius morosus (Br.)

Publication Type:Journal Article
Year of Publication:1994
Authors:Cecchettini, A
Journal:Bolletino di zoologia
Volume:61
Issue:4
Date Published:1994
ISBN Number:0373-4137
Keywords:Fringillidae, Serinus, Serinus serinus
Abstract:Abstract Two distinct proteolytic activities were demonstrated in developing ovarian follicles of the stick insect Carausius morosus using aldolase as a substrate. One has an optimum activity around pH 5 and it is strongly inhibited by pepstatin A. Because of these properties, it is classifiable as an aspartic proteinase. The second one has an alkaline pH optimum and it is inhibited by aprotinin. Based on these properties it is classifiable as a serine, trypsin?like, proteinase. This latter activity has also been visualized as a negatively stained protein fraction of about 26 kD on polyacrylamide gels containing casein as substrate. While the aspartic proteinase is predominantly associated with follicles approaching chorionogenesis, the serine proteinase is restricted to immature follicles and disappears prior to completion of ovarian development.Abstract Two distinct proteolytic activities were demonstrated in developing ovarian follicles of the stick insect Carausius morosus using aldolase as a substrate. One has an optimum activity around pH 5 and it is strongly inhibited by pepstatin A. Because of these properties, it is classifiable as an aspartic proteinase. The second one has an alkaline pH optimum and it is inhibited by aprotinin. Based on these properties it is classifiable as a serine, trypsin?like, proteinase. This latter activity has also been visualized as a negatively stained protein fraction of about 26 kD on polyacrylamide gels containing casein as substrate. While the aspartic proteinase is predominantly associated with follicles approaching chorionogenesis, the serine proteinase is restricted to immature follicles and disappears prior to completion of ovarian development.
URL:http://www.tandfonline.com/doi/abs/10.1080/11250009409355898
Short Title:Bolletino di zoologia
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith