AVIS-IBIS

Birds of Indian Subcontinent

High levels of polymorphism detected with M13 fingerprinting probe in bird species and populations

Publication Type:Journal Article
Year of Publication:1998
Authors:FULGIONE, DOMENICO, Procaccini, G, Varriale, B, Pascucci, B, Milone, M
Journal:Italian Journal of Zoology
Volume:65
Issue:3
Date Published:1998
ISBN Number:1125-0003
Keywords:Columba, Columba palumbus, Columbidae, Passer, Passer montanus, Passeridae
Abstract:Abstract Hypervariable minisatellite markers have widely been used in forensic, paternity, and mating system studies, and applied to population genetics of animals and plants. In birds, minisatellite DNA fingerprinting was first developed through the use of 33.15 and 33.6 poly?core probes. To date, few studies exist where other fingerprinting probes have been used. The aim of the present analysis is to extend the use of the M13 minisatellite probe to the genetic study of natural populations of passerine and non?passerine birds through the optimization of an easy?to?make procedure, in order to address questions related with the biogeography, ecology, reproductive biology, and systematics of this group. The M13 fingerprinting probe yielded multibanded fingerprints from the genomes of all species analysed, and the average number of bands was comparable with values obtained in literature using different multilocus probes. Similarity values varied according to the phylogenetic distances among taxonomic units. In Passer montanus, the analysed populations proved to be genetically distinct, and the within?population similarity values were comparable with those obtained, using the same technique, in a congeneric species.Abstract Hypervariable minisatellite markers have widely been used in forensic, paternity, and mating system studies, and applied to population genetics of animals and plants. In birds, minisatellite DNA fingerprinting was first developed through the use of 33.15 and 33.6 poly?core probes. To date, few studies exist where other fingerprinting probes have been used. The aim of the present analysis is to extend the use of the M13 minisatellite probe to the genetic study of natural populations of passerine and non?passerine birds through the optimization of an easy?to?make procedure, in order to address questions related with the biogeography, ecology, reproductive biology, and systematics of this group. The M13 fingerprinting probe yielded multibanded fingerprints from the genomes of all species analysed, and the average number of bands was comparable with values obtained in literature using different multilocus probes. Similarity values varied according to the phylogenetic distances among taxonomic units. In Passer montanus, the analysed populations proved to be genetically distinct, and the within?population similarity values were comparable with those obtained, using the same technique, in a congeneric species.
URL:http://www.tandfonline.com/doi/abs/10.1080/11250008809386760
Short Title:Italian Journal of Zoology
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith