AVIS-IBIS

Birds of Indian Subcontinent

DNA Double-Strand Breaks and γ-H2AX Signaling in the Testis1

Publication Type:Journal Article
Year of Publication:2003
Authors:Hamer, G, Roepers-Gajadien, HL, van Duyn-Goedhart, A, Gademan, IS, Kal, HB, van Buul, PPW, de Rooij, DG
Journal:Biology of Reproduction
Volume:68
Issue:2
Date Published:2003
ISBN Number:0006-3363
Keywords:Fringillidae, Serinus, Serinus serinus
Abstract:Abstract Within minutes of the induction of DNA double-strand breaks in somatic cells, histone H2AX becomes phosphorylated at serine 139 and forms ?-H2AX foci at the sites of damage. These foci then play a role in recruiting DNA repair and damage-response factors and changing chromatin structure to accurately repair the damaged DNA. These ?-H2AX foci appear in response to irradiation and genotoxic stress and during V(D)J recombination and meiotic recombination. Independent of irradiation, ?-H2AX occurs in all intermediate and B spermatogonia and in preleptotene to zygotene spermatocytes. Type A spermatogonia and round spermatids do not exhibit ?-H2AX foci but show homogeneous nuclear ?-H2AX staining, whereas in pachytene spermatocytes ?-H2AX is only present in the sex vesicle. In response to ionizing radiation, ?-H2AX foci are generated in spermatogonia, spermatocytes, and round spermatids. In irradiated spermatogonia, ?-H2AX interacts with p53, which induces spermatogonial apoptosis. These events are independent of the DNA-dependent protein kinase (DNA-PK). Irradiation-independent nuclear ?-H2AX staining in leptotene spermatocytes demonstrates a function for ?-H2AX during meiosis. ?-H2AX staining in intermediate and B spermatogonia, preleptotene spermatocytes, and sex vesicles and round spermatids, however, indicates that the function of H2AX phosphorylation during spermatogenesis is not restricted to the formation of ?-H2AX foci at DNA double-strand breaks.Abstract Within minutes of the induction of DNA double-strand breaks in somatic cells, histone H2AX becomes phosphorylated at serine 139 and forms ?-H2AX foci at the sites of damage. These foci then play a role in recruiting DNA repair and damage-response factors and changing chromatin structure to accurately repair the damaged DNA. These ?-H2AX foci appear in response to irradiation and genotoxic stress and during V(D)J recombination and meiotic recombination. Independent of irradiation, ?-H2AX occurs in all intermediate and B spermatogonia and in preleptotene to zygotene spermatocytes. Type A spermatogonia and round spermatids do not exhibit ?-H2AX foci but show homogeneous nuclear ?-H2AX staining, whereas in pachytene spermatocytes ?-H2AX is only present in the sex vesicle. In response to ionizing radiation, ?-H2AX foci are generated in spermatogonia, spermatocytes, and round spermatids. In irradiated spermatogonia, ?-H2AX interacts with p53, which induces spermatogonial apoptosis. These events are independent of the DNA-dependent protein kinase (DNA-PK). Irradiation-independent nuclear ?-H2AX staining in leptotene spermatocytes demonstrates a function for ?-H2AX during meiosis. ?-H2AX staining in intermediate and B spermatogonia, preleptotene spermatocytes, and sex vesicles and round spermatids, however, indicates that the function of H2AX phosphorylation during spermatogenesis is not restricted to the formation of ?-H2AX foci at DNA double-strand breaks.
URL:http://dx.doi.org/10.1095/biolreprod.102.008672
Short Title:Biology of Reproduction
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith