AVIS-IBIS

Birds of Indian Subcontinent

Use of Monoclonal Antibodies against Chicken Coccidia to Study Invasion and Early Development of Eimeria gruis in the Florida Sandhill Crane (Grus canadensis)

Publication Type:Journal Article
Year of Publication:1998
Authors:Augustine, PC, Klein, PN, Danforth, HD
Journal:Journal of Zoo and Wildlife Medicine
Volume:29
Issue:1
Date Published:1998
ISBN Number:10427260
Keywords:Gruidae, Grus, Grus canadensis, Grus grus, Meleagris, Meleagris gallopavo, Phasianidae
Abstract:Eimeria gruis and E. reichenowi are common coccidial parasites of a number of crane species. In the present study, monoclonal antibodies (McAbs), elicited against Eimeria spp. of chickens and turkeys, cross-reacted with sporozoites and developmental stages of E. gruis in the tissues of Florida sandhill cranes. These McAbs were used to define the area of the intestine that was invaded by sporozoites of E. gruis and to demonstrate the feasibility of using McAbs to study the early development of E. gruis in the intestines and visceral organs of cranes. At 6 hr postinoculation (PI), E. gruis sporozoites were found primarily from just proximal to Meckle's diverticulum in the jejunum to the ileocecal juncture. Fewer sporozoites were found in the ceca and rectum, and none were found in the duodenum. Most of the sporozoites were in the middle third of the villi and within the lamina propria. At 14 days PI, developmental stages were detected in the ceca, jejunum, liver, and lungs but not in the heart, kidney, or brain. In the ceca and jejunum, the number, location, and maturity of the stages differed markedly.
URL:http://www.jstor.org/stable/20095710
Short Title:Journal of Zoo and Wildlife Medicine
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