AVIS-IBIS

Birds of Indian Subcontinent

Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba

Publication Type:Journal Article
Year of Publication:2011
Authors:Luttikhuizen, P, Bol, A, Witte, H, van Bleijswijk, J, Haddrath, O, Baker, A, Piersma, T, Reneerkens, J
Journal:Journal of Ornithology
Volume:152
Issue:3
Date Published:2011
ISBN Number:2193-7192
Keywords:Calidris, Calidris alba, Calidris melanotos, Crocethia alba, Ereunetes albus, Ereunetes melanotos, Greenland, Scolopacidae, Xenus, Xenus cinereus
Abstract:We isolated and tested six novel microsatellite loci in Sanderling ( Calidris alba ) from Greenland for paternity analyses. In addition, we tested 11 already published microsatellite markers which were originally developed for the congeneric species, the Pectoral Sandpiper ( C. melanotos ). All loci were polymorphic, but five of the cross-species loci were not scorable due to suboptimal amplification patterns. The 12 successful loci were tested on 87 individuals, yielding an average of 9.0 (range 4–19) alleles per locus and mean expected heterozygosity of 0.70. Because this dataset contained families, tests for Hardy–Weinberg equilibrium, linkage disequilibrium and probability of identity were done on a subset of the data containing 25 adults caught in the same year. The overall probability of identity was 1.0 × 10 −13 . Only one locus displayed significant homozygote excess and all loci were unlinked. On the basis of female heterozygotes, all loci are assumed to be autosomal.
URL:http://dx.doi.org/10.1007/s10336-011-0681-6
Taxonomic name: 
Calidris (Birds), Calidris alba (Birds), Calidris melanotos (Birds), Scolopacidae (Birds), Xenus (Birds), Xenus cinereus (Birds)
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith